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Cells were removed by centrifugation at 3270 rcf for 10 min at 4 °C in a bench top centrifuge (Allegra X-12R, Beckman Coulter) followed by filtration through a 1000 cm 2 , 0.8/0.2 µm filter (12992, Pall) to create C-HCCF
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[3] [4] The mechanism of action centres on B12's essential role as a cofactor in cellular metabolism
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