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Physiological shear-stress levels on the EC layer were achieved by asymmetrically tilting the platform (fast tilting to the left and slow tilting back to the horizontal position) to generate a hydrostatic pressure difference between the medium reservoirs and to obtain quasi-unidirectional flow, which increased the gas and nutrient exchange with the medium and helped to avoid potential hypoxic conditions as a consequence of the low gas permeability of the plastic material

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