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The samples were then transferred to DAB destaining solution (glycerol:acetic acid:ethanol at 1:1:3) and incubated at 23 °C with gentle shaking until entirely destained
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Once outside the cell, GSH is cleaved into Cys-Gly by the enzyme γ -glutamyl transpeptidase (γ-GT), which is located on the astrocytic cell membrane
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