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Another meta-analysis, which included 11,049 patients, demonstrated that carboplatin, combined with dual HER2 blockade (pertuzumab + trastuzumab) plus docetaxel, was more effective than the same regimen without carboplatin in neoadjuvant treatment for HER2-positive breast cancer [63]
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Cells were incubated with the relevant antibody in blocking buffer for 1 h at room temperature, washed 3 times with 1× PBS and were incubated with secondary antibodies for 1 h
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