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Beads were captured on a magnetic stand, the supernatant was discarded, and the beads were sequentially washed with 1 mL each of wash buffers 1 (2% SDS), 2 (20 mm Tris–HCl [pH 8], 500 mm NaCl, 2 mm EDTA, 0.1% deoxycholate, 0.1% (wt/vol) SDS, and 1% (vol/vol) Triton X-100), 3 (10 mm Tris–HCl [pH 8], 250 mm LiCl, 1 mm EDTA, 1% [vol/vol] NP-40%, and 0.5% [wt/vol] sodium deoxycholate), and 4 (10 mm Tris–HCl [pH 8], 1 mm EDTA), with each wash lasting 8 min at room temperature