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Conditions that should be optimized to facilitate refolding include: type of denaturant, pH, presence of reducing agent, speed of denaturant removal, and protein concentration

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[DOI] [PMC free article] [PubMed] [Google Scholar] 114.Bartels ED, Guo S, Kousholt BS, Larsen JR, Hasenkam JM, Burnett J, Jr., et al