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Glutathione Modified Fluorescent CdS QDs Synthesized Using Environmentally Benign Pathway for Detection of Mercury Ions in Aqueous Phase | Journal of Fluorescence | Springer Nature Link

Before exposure, medium was exchanged for HBSS, and cells were exposed to 37°C, 3.5L/minute 5% CO 2 /air + 350ppb O 3 for 1 hour, then allowed to recover for 24 hours in fresh medium

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However, someone with a more severe deficiency might need to wait a little longer, possibly requiring multiple injections to replenish their B12 stores