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The transfection protocol involved seeding NPCs in 6-well plates to 60–70% confluence, then incubating the cells for 6 h with a transfection complex comprising 50 nM siRNA, Opti-MEM, and Lipofectamine 2,000 followed by replacing the medium with growth medium and culturing for 72 h
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These therapeutic peptides are being studied for their ability to cross the blood-brain barrier, support neural repair, improve synaptic communication, and reduce oxidative stress and inflammation key contributors to brain degeneration
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