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To monitor cell viability, the cells were trypsinized, and apoptosis/necrosis induced by pneumococcal infection was evaluated using flow cytometry with an Annexin V/propidium iodide labeling kit (Invitrogen), showing ∼5% across all time points

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It is ubiquitously expressed in many tissues and mainly distributed in the cytosol and mitochondria of the erythrocytes, liver, lungs, and kidney [7,8]