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Cell viability was assessed 3 days after treatment with FOXO4-DRI at 0, 12.5, 25, 50, and 100 μM, respectively, using the Cell Counting Kit-8 (CCK-8) Assay (Dojindo, Rockville, MD, United States)

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Step 7: Sterile Filtration For cell culture applications, filter the reconstituted peptide through a 0.22 μm sterile filter

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