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After washing, the sections were further incubated with the goat anti-rabbit IgG (H + L) secondary antibody, HRP (#EF0002, Sparkjade, 1:200 dil) and goat anti-mouse secondary antibody, HRP (#EF0001, Sparkjade, 1:200 dil) for 1 h at room temperature, respectively

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Tip: Seal the pouch tightly after use to prevent pads from drying out