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The cells were incubated for 1 h in a blocking solution with a combination of anti-p62 (1:400), anti-ubiquitin (1:400), anti-LC3B (1:400), and anti-lysosomal-associated membrane protein 1 (LAMP1, 1:400) antibodies at 4°C in a humidified chamber

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doi: 10.1038/sj.mp.4001790